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ETD-MS2/MS3 comparison of glycosylated and non-glycosylated CH3 peptide amino acids 360–369 after treatment with endo-F2. The modified [M + 2H]2+ peptide ion at m/z = 756.4 Da was first analyzed by CID-MS2 to remove the core fucose. The dominant [M + 2H]2+ product ion a at m/z = 683.4 Da was analyzed by ETD-MS3 and the resulting spectrum was compared with the ETD-MS2 spectrum from the unmodified [M + 2H]2+ peptide ion at m/z = 581.9 Da.
