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ETD-MS2/MS3 comparison of glycosylated and non-glycosylated VL peptide amino acids 103–109 after treatment with endo-F2. The modified [M + 2H]2+ peptide ion at m/z = 557.8 Da was first analyzed by CID-MS2 to remove the core fucose. The dominant [M + 2H]2+ product ion a at m/z = 485.3 Da was subsequently analyzed by ETD-MS3 and the resulting spectrum was compared with the ETD-MS2 spectrum from the unmodified [M + 2H]2+ peptide ion at m/z = 383.8 Da.
