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In situ FITC-gelatin zymography. MCF7-mock, MCF7-MT1, and MCF7-MT6F cells were seeded in serum-free DMEM on FITC-gelatin-coated coverslips. In 16 h, cells were fixed, and the nuclei were stained with DAPI. The slides were observed using a fluorescent microscope (magnification, ×400).
