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Rapid activation of GCL following oxidative stress. Jurkat cells were treated with phorone for (90 min) or H2O2 (10 min) and GCL activity (A) and cellular GSH levels (B) were measured. (Mean percentage of control activity ± S.E. are shown; *, statistically different from control by Wilcoxon signed rank test (p < 0.05)). C, immunoblot analysis of GCLC protein in extracts of Jurkat cells treated with H2O2 (left lanes) or phorone (right lanes).
