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. 2010 Mar 23;285(21):16116–16124. doi: 10.1074/jbc.M110.116210

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Disulfide bond formation and high molecular weight forms of GCL. A, extensive formation of high molecular weight complexes in frozen extracts from mouse liver (Li), kidney (K), and brain (B), but not in control (Ly) and phorone-treated Jurkat cells (LyP). Samples were resolved by SDS-PAGE under non-reducing or reducing conditions and stained for GCLC and GCLM. B, in fresh rat kidney and liver, prepared with or without 5 mm DTT, high molecular weight form is a minor component of GCLC. Samples were resolved by SDS-PAGE under non-reducing or reducing conditions and stained for GCLC.