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BRET methodology. a, shown is a schematic representing ligand-dependent dimerization and resonance energy transfer between RLuc and YFP fusions via BRET. b, the BRET ratio is calculated as the ratio of YFP emission to RLuc emission with subtraction of the background and a correction factor for emission spectrum isolation. c, ERα and ERβ BRET fusions localize to the nucleus, in contrast with the cytoplasmic presence of pCMX-YFP.
