FIGURE 3.
a, Hsp90 inhibition ablated the transcriptional activity of ERα and ERβ. Overnight 17-DMAG treatment inhibited E2-mediated activation of a pERE-Luc reporter in the presence of all three dimer pairs. Error bars represent mean ± S.D. *, statistically significant increase in transcriptional activity compared with DMSO (left bars) or 17-DMAG alone (right bars). RLU, relative luciferase units. b, co-immunoprecipitations showed that Hsp90 interacted with ERα (left panel) and ERβ (right panel), and this interaction was disrupted by treatment with 10 nm E2, 250 nm 17-DMAG, and the combination of both ligands.