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. 2010 Mar 16;285(21):16248–16257. doi: 10.1074/jbc.M109.048785

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — NUP98-HOXA9 and NUP98-DDX10 co-immunoprecipitate with endogenous CRM1. A, K562 cells were nucleofected with either empty pcDNA3 vector (Control) or vector expressing FLAG-tagged NUP98-HOXA9 or NUP98-DDX10. Anti-NUP98 immunoblot of nuclear lysates shows the transduced fusion proteins as well as endogenous NUP98. B, nuclear lysates and immunoprecipitates (IP) prepared using anti-FLAG antibody from control cells or cells expressing the indicated FLAG-tagged NUP98 fusions were analyzed by anti-CRM1 immunoblotting.