. 2010 Mar 11;285(21):16286–16293. doi: 10.1074/jbc.M110.108167

TABLE 3.

ITC measurements of E2F^TD binding to mutants of phosRb^380–787

Construct	Mutations	Accessible phosphorylation sites	E2F^TDK_d
unphosRb^380–787	None	NA^a	0.045 ± 0.007 μm
phosRb^380–787	None	Ser⁶⁰⁸, Ser⁶¹², Ser⁷⁸⁰	0.7 ± 0.4 μm
phosRb^{380–787Δ604}	D604A	Ser⁶⁰⁸, Ser⁶¹², Ser⁷⁸⁰	0.4 ± 0.2 μm
phosRb^{380–787Δ605}	M605A	Ser⁶⁰⁸, Ser⁶¹², Ser⁷⁸⁰	0.9 ± 0.2 μm
phosRb^{380–787Δ606}	Y606A	Ser⁶⁰⁸, Ser⁶¹², Ser⁷⁸⁰	0.22 ± 0.09 μm
phosRb^{380–787Δ604/605/606}	D604A, M605A, Y606A	Ser⁶⁰⁸, Ser⁶¹², Ser⁷⁸⁰	0.11 ± 0.05 μm
phosRb^{380–787Δ607}	L607A	Ser⁶⁰⁸, Ser⁶¹², Ser⁷⁸⁰	0.3 ± 0.2 μm
phosRb^{380–787Δ608}	S608A	Ser⁶¹², Ser⁷⁸⁰	0.15 ± 0.01 μm
phosRb^{380–787Δ612}	S612A	Ser⁶⁰⁸, Ser⁷⁸⁰	0.7 ± 0.1 μm
phosRb^{380–787Δ608/612}	S608A, S612A	Ser⁷⁸⁰	0.06 ± 0.04 μm

^a Not applicable.