FIGURE 1.

GM130 contains a C-terminal PDZ ligand. A, schematic of the C termini of GM130 through evolution from its first appearance in Drosophila melanogaster to Homo sapiens. B, purified myristoylated G65-His (2 μg) incubated with the GST-tagged GM130 C terminus with or without an appended arginine (2 μg). Recovery on glutathione-agarose beads was determined by immunoblotting (anti-His) and staining with Ponceau S. Binding was quantified (n = 3, ±S.E. (error bars)).