TABLE 2.
Linkage analysis of partially methylated alditol acetate derivatives of the PNGase-F-released N-glycans from both MPO forms
| Assignments | Characteristic fragment ions | Elution time | Relative abundance |
|
|---|---|---|---|---|
| h-MPO | r-MPO | |||
| min | ||||
| Terminal fucose | 115,118,131,162,175 | 14:00 | 0.169 | 0.005 |
| Terminal mannose | 102,118,129,145,161,162,205 | 18:33 | 0.557 | 1.324 |
| Terminal galactose | 102,118,129,145,161,162,206 | 19:25 | 0.079 | 0.558 |
| 2-Linked mannose | 129,130,161,190,234 | 22:31 | 1.000 | 1.000 |
| 3-Linked galactose | 101,118,129,161,234 | 23:22 | 0.154 | 0.171 |
| 6-Linked galactose | 118,162,189,233 | 25:19 | 0.135 | - |
| 2,4-Linked mannose | 130,190,233 | 26:35 | 0.028 | 0.035 |
| 2,6-Linked mannose | 129,130,189,190 | 28:09 | 0.113 | - |
| 3,6-Linked mannose | 118,129,189,234 | 28:32 | 0.223 | 1.059 |
| Terminal GlcNAc | 117,159,203,205 | 31:06 | 0.119 | 0.121 |
| 4-Linked GlcNAc | 117,159,233 | 33:31 | 0.509 | 1.246 |
| 4,6-Linked GlcNAc | 117,159,261 | 37:00 | 0.007 | 0.043 |