TABLE 1.
Bacterial strains, yeast strains, and plasmids used in this study
| Strain/plasmid | Relevant characteristics | Source/Ref. |
|---|---|---|
| E. coli | ||
| DH5α | supE44 Δ lacU169 (Ø80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | (40) |
| BL21 (DE3) | F−ompT gal dcm lon hsdSB (rB− mB−; E. coli B strain), with DE3, a λ prophage carrying the T7 RNA pol gene | Stratagene |
| S. enterica | ||
| 14028 | Wild type | ATCC |
| SV5193 | 14028 slrP::3xFLAG, Kmr | (12) |
| S. cerevisiae | ||
| AH109 | MATa, trp1–901, leu2–3, 112, ura3–52, his3–200, gal4Δ, gal80Δ, LYS2::GAL1UAS-GAL1TATA-HIS3, GAL2UAS-GAL2TATA-ADE2 URA3:: MEL1UAS-MEL1TATA-LacZ MEL1 | A. Holtz, unpublished |
| Y187 | MATα, ura3–52, his3–200, ade2–101, trp1–901, leu2–3, 112, gal4Δ, gal80Δ, met–, URA3:: GAL1UAS-GAL1TATA-LacZ MEL1 | (41) |
| Plasmids | ||
| pcDNA3 | Transient or stable transfection vector, Ampr | Invitrogen |
| pCS2+HA3 | Transient transfection vector, Ampr | F. Romero |
| pGADT7 | GAL4(768–881) AD, LEU2, Ampr | Clontech |
| pGBT10 | GAL4(1–147) DB, TRP1, Ampr | Clontech |
| pGEX-4T-1 | GST fusion vector, Ampr | Pharmacia |
| pGEX-4T-3 | GST fusion vector, Ampr | Pharmacia |
| pIZ1623 | pGEX-4T-3-SlrP | (12) |
| pIZ1627 | pGBT10-SlrP | (12) |
| pIZ1706 | pGADT7-ERdj3, clone obtained in the two-hybrid screen | This study |
| pIZ1708 | pGEX-4T-1-ERdj3 | This study |
| pIZ1720 | pCS2-SlrP-3xFLAG | (12) |
| pIZ1725 | pcDNA3-SlrP-3xFLAG | (12) |
| pIZ1729 | pCS2-ERdj3–3xHA | This study |
| pIZ1780 | pcDNA3-SlrP(C546A)-3xFLAG | (12) |
| pIZ1791 | pGADT7-ERdj3(23–358) | This study |
| pIZ1792 | pGADT7-ERdj3(88–358) | This study |
| pIZ1793 | pGADT7-ERdj3(129–358) | This study |
| pIZ1794 | pGADT7-ERdj3(160–358) | This study |
| pIZ1795 | pGADT7-ERdj3(201–358) | This study |
| pIZ1796 | pGADT7-ERdj3(251–358) | This study |
| pIZ1797 | pGEX-4T-1-ERdj3(23–358) | This study |
| pIZ1798 | pGEX-4T-1-ERdj3(88–358) | This study |
| pIZ1799 | pGEX-4T-1-ERdj3(129–358) | This study |
| pIZ1800 | pGEX-4T-1-ERdj3(160–358) | This study |
| pIZ1801 | pGEX-4T-1-ERdj3(201–358) | This study |
| pIZ1802 | pGEX-4T-1-ERdj3(251–358) | This study |
| pIZ1804 | pGBT10-SlrP(C546A) | This study |
| pQE10-BiP | L. Hendershot | |