TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide/use | Sequence 5′-3′ (restriction sites are underlined) |
|---|---|
| Construction of pIZ1708 | |
| dnaj5′ | ATGCGAATTCATGGCTCCGCAGAACCTGAG |
| dnaj3′ | TGACCTCGAGGTCCAATTTTATTCACTCTC |
| Construction of pIZ1729 | |
| 3HA5′ | ATCGCTCGAGTACCCATACGATGTTCC |
| 3HA3′ | TGCATCTAGATCAGCCAGCGTAGTCTGGTAC |
| Dnajbam5′ | CTGAGGATCCACCATGGCTCCGCAGAACCTGAG |
| Dnajxho3′ | TGACCTCGAGATATCCTTGCAGTCCATTGTATAC |
| Construction of pIZ1791 and pIZ1797 | |
| dnaj23eco5′ | CATGGAATTCGGACGAGATTTCTATAAGATC |
| dnaj3′ | as above |
| Construction of pIZ1792 and pIZ1798 | |
| dnaj88eco5′ | CATGGAATTCACTTATGGTGAAGAAGGATTAAAAG |
| dnaj3′ | as above |
| Construction of pIZ1793 and pIZ1799 | |
| dnaj129eco5′ | CATGGAATTCCCAAGAGGAAGTGATATTATTG |
| dnaj3′ | as above |
| Construction of pIZ1794 and pIZ1800 | |
| dnaj160eco5′ | GATCGAATTCGCAAGGCAGGCTCCTGGCAAAC |
| dnaj3′ | as above |
| Construction of pIZ1795 and pIZ1801 | |
| dnaj201eco5′ | CATGGAATTCCTAGTGAATGAAGAACGAAC |
| dnaj3′ | as above |
| Construction of pIZ1796 and pIZ1802 | |
| dnaj251eco5′ | CATGGAATTCTTTGAAAGGAGAGGAGATGATTTG |
| dnaj3′ | as above |
| Sequencing of pGADT7 derivatives | |
| T7 | TAATACGACTCACTATAGGG |
| Sequencing of pGEX derivatives | |
| pgex5′ | GGGCTGGCAAGCCACGTTTGGTG |
| pgex3′ | CCGGGAGCTGCATGTGTCAGAGG |