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A, PCR screening in tail genomic DNA from PNMTCre/FloxedGRK2 (PNMTCre/Flox) or control FloxedGRK2 (Flox) mice for confirmation of PNMT locus insertion of the Cre transgene. An additional lane run without DNA (H2O), thus serving as negative control for the assay is also shown. B, real-time PCR in total adrenal mRNA isolated from 2-month-old PNMT-driven GRK2 KO or control FloxedGRK2 (WT) mice for determination of total adrenal GRK2 mRNA levels (*, p < 0.05, n = 6 mice/litter).
