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. Author manuscript; available in PMC: 2011 May 12.
Published in final edited form as: J Am Chem Soc. 2010 May 12;132(18):6474–6480. doi: 10.1021/ja1007849

Figure 3.

Figure 3

Normalized steady-state fluorescence excitation and emission spectra of equilenin. Emission from KSI bound equilenin at room temperature (solid, black) and 77 K (solid, blue) was observed from 350 nm and 365 nm excitation, respectively. Corresponding excitation spectra (broken line with matching color) were observed from 400 nm emission. Emission from equilenin in buffer at room temperature (solid, red) and 77 K (solid, purple) was observed from excitation at 360 nm and 350 nm, respectively. Excitation spectra (broken lines) of the room temperature sample (red) were observed from emission at 420 nm while the 77 K sample (purple) was observed from 390 nm emission. The protein-bound spectra were taken in pH 7, 40 mM potassium phosphate buffer while the unbound spectra were determined in 0.5 M NaOH. All spectra were obtained in 50% glycerol.