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. Author manuscript; available in PMC: 2010 May 17.
Published in final edited form as: J Cell Physiol. 2009 Feb;218(2):385–393. doi: 10.1002/jcp.21610

FIGURE 3. No Effect of Rac1DN On Kir2.1 Single-Channel Properties.

FIGURE 3

A) Representative single-channel traces from control plasmid (Ctrl) or Rac1DN transfected cells in cell-attached patch clamp recordings at various voltages. The dashed line represents the closed state of the channel and the solid line represents the open state. B) Current-voltage relationship of single-channel currents from control (filled squares) and Rac1DN transfected cells (open squares). Lines represent linear fits to the data points; the solid line is the fit to control cells, the dashed line is the fit to Rac1DN transfected cells. Insets: Slope conductance calculated from linear fit to current amplitudes of individual cells using three or more data points. C) Open probability of Kir2.1 in control and Rac1DN transfected cells at –40 mV. D) Mean open and E) closed dwell times of control and Rac1DN transfected cells at –40 mV. Significance was tested using non-parametric t-tests. Data was obtained from 5-6 cells per condition.