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. Author manuscript; available in PMC: 2010 May 17.
Published in final edited form as: Glia. 2007 Apr 1;55(5):453–462. doi: 10.1002/glia.20467

Fig. 7.

Fig. 7

Delayed and time-dependent loss of nigral dopaminergic neurons following LPS i.p. injection. Male C57BL/6 mice were treated with saline or LPS (5 mg/kg, i.p.) and then maintained under normal conditions for the indicated time points. Mice were sacrificed and brain sections (35 mm thick) were cut through the nigral complex. After immunostaining with TH antibody, the number of TH-IR neurons in the SN was counted as described in methods. Results are expressed as percentage of the corresponding saline controls. (A) Number of TH-IR neurons in the SN of control and LPS-treated mice at different time points. (B) Visualization of TH-IR neurons in the substantia nigra and ventral tegmental area of saline and LPS-treated animals at 10 months. (C) F4/80-Immunohistochemistry for microglia within the SN and VTA of saline and LPS treated animals at 10 months. Note the high density of F4/80-IR microglia in the substantia nigra associated with the loss of dopaminergic neurons in mice treated with LPS. **P < 0.01, compared to the saline controls (n = 8–10 per time point).