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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Bioanal Rev. 2009 Dec;1(1):7–24. doi: 10.1007/s12566-009-0001-2

Fig. 5.

Fig. 5

HPLC separations of enkephalin diastereomers on (A) teicoplanin stationary phase compared to (B) C18 stationary phases. The arrow in (A) indicates the beginning of a step gradient (from isocratic 78% ACN/22% 3 mM ammonium acetate to 70% CAN/30% 0.1% (v/v) formic acid in water at 36 min) found necessary to decrease the elution time of E3 and E4. Arrows in (B) indicate the linear gradient in between (80% 13 mM ammonium acetate/20% ACN at 10 min to 30% ACN at 45min). These two stationary phases are different in selectivity and thus peptides have different retention orders. Enantiomeric pair E0 and E4 and diastereomeric pair E1a and E3were not separated on the non-chiral column, whereas teicoplanin-based CSP was able to resolve every peptide. Adapted with permission from Springer [69].