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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Bioanal Rev. 2009 Dec;1(1):7–24. doi: 10.1007/s12566-009-0001-2

Fig. 9.

Fig. 9

Characterization of DAACPs from a complex mixture of peptides using an enzymatic digestion method. Aplysia abdominal ganglia were used to make extract. (A) and (B) are LC base peak chromatograms showing 465–466 m/z range, which matches the NWFa/NdWFa peak, without enzyme treatment (A) or with 1.0 units of mAAP treatment (B). (C) and (D) are corresponding MS spectra showing NWFa/NdWFa peaks. Enzymatic treatment facilitated detection of otherwise barely detectable peptides. Reproduced with permission from the American Chemical Society [113].