Figure 2.
Tolerance of OmniTaq (OT) and Omni Klentaq (OKT) to blood treated with different anticoagulants. Two endogenous targets, 300 bp of the β-actin gene (A) and 488 bp of the HRES1 gene (B), were amplified from 1.25 to 25% (of total PCR volume) from a whole blood sample treated with citrate, heparin, or EDTA, separately, using OT or OKT in the absence (W/O) or presence (W/W) of PEC. The positive control contained 4 ng of purified DNA (0). The products were analyzed in 1.5% agarose gel electrophoresis. Lanes M, DNA standards ladder.