Table 1.

Primers Listed by Assay

Fragment analysis
A1	EGFR-Ex19-FW1: 5′-GCACCATCTCACAATTGCCAGTTA-3′
A2	EGFR-Ex19-REV1: 5′-Fam-AAAAGGTGGGCCTGAGGTTCA-3′
B1	EGFR-Ex 19-FW2: 5′-ATCCCAGAAGGTGAGAAAGATAAAATTC-3′
B2	EGFR-Ex 19-REV2: 5′-CCTGAGGTTCAGAGCCATGGA-3′
B3	EGFR-Ex 19-REV3: 5′-Fam-CCTGAGGTTCAGAGCCATGGA-3′
C1	EGFR-Ex 19-FW4: 5′-CCCAGCAATATCAGCCTTAGGTG-3′
C2	EGFR-Ex 19-REV4: 5′-CCACTAGAGCTGGAAAGGGAAAGA-3′
D1	EGFR-Ex21-FW1: 5′-CCTCACAGCAGGGTCTTCTCTGT-3′
D2	EGFR-Ex21-REV1: 5′-Fam-TCAGGAAAATGCTGGCTGACCTA-3′
Sequenom Mass Spectrometry assay
E1	2573-FW 5′-ACGTTGGATGACGTACTGGTGAAAACACCG-3′
E2	2573- REV: 5′-ACGTTGGATGTCTTTCTCTTCCGCACCCAG-3′
E3	2573 extension primer-REV: 5′-CACCCAGCAGTTTGGCC-3′
	Mass of extension primer: 5131.3 Da
	Mass of extension product Call T: 5402.6 Da
	Call G: 5378.5 Da