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. 2010 Mar;12(2):226–237. doi: 10.2353/jmoldx.2010.090042

Figure 1.

Figure 1

A: Schematic representation of the TCR-γ genomic DNA locus. Approximate positions of the primers for both mono-V and multi-V PCR strategies are marked by arrows. The bars represent consensus primer-binding sequences. * ← JP primer is only used in the mono-V assay. B:TCR-γ variable gene segments V1-8, V9, V10, V11 (forward primers) and J segments (reverse primers): Reference DNA sequences from GenBank (top), mono-V TCR-γ primers (middle), multi-V TCR-γ primers (bottom). All DNA sequences are in 5′ to 3′ orientation. C: Detection of TCR-γ PCR products by capillary gel electrophoresis. Mono-V (left column) and multi-V (right column) PCR assays amplified representative DNA samples from the same patient.