Figure 3.
Effect of combining individual multi-V TCR-γ V9 and V11 BIOMED-2 primers. We synthesized multi-V TCR-γ V9 and V11 primers based on their published DNA sequences.15 We added the primers individually (A, D, G and B, E, H) and in combination with each other (C, F, I) to amplify TCR-γ GRs from control DNA. Reactive tonsil DNA (A–C), 100% Jurkat T-cell line DNA (D–F), and 10% Jurkat T-cell line DNA diluted in reactive tonsil DNA (G–I) were used at 1 μg in 50 μl reaction volume. All PCR reactions included a mixture of J1/2 and JP1/P2 primers.