Table 3.
Clinical and Molecular Features of Patients with Clonal TCR-γ V11 Gene Rearrangements
|
TCR-β |
TCR-γ primer sets |
IGH |
||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mono-V |
Multi-V |
FR2 | FR3 | |||||||||||||||||
| A | B | C | V1-8 | V9 | V10 | V11 |
V1-8 |
V9 |
V10 |
V11 |
||||||||||
| TA 60°C |
TA 55°C |
TA 60°C |
||||||||||||||||||
| Patient no. | Age/ sex | DNA per reaction (ng) | Final diagnosis | JP1/JP2 | JP | J1/J2 | No Added MgCl2* | +1 mM MgCl2† | ||||||||||||
| 1 | 49/M | 33 | CTCL | + | + | + | + | +‡ | − | ND | − | ND | + | +‡ | − | − | ND | ND | − | − |
| 2 | 84/F | 220 | CTCL | + | − | + | − | − | − | + | − | − | − | − | − | − | + | − | NT | NT |
| 3 | 59/F | 245 | CTCL | − | − | + | + | − | − | + | − | − | + | − | − | − | + | + | NT | NT |
| 4 | 72/F | 175 | CTCL | − | − | − | + | − | − | ND | ND | ND | + | − | − | − | ND | ND | NT | NT |
| 5 | 55/F | 190 | CTCL | − | + | +‡ | + | − | − | + | − | − | + | − | − | − | + | +‡ | NT | NT |
| 6 | 80/F | 305 | T/NKL | − | − | − | − | − | − | + | − | + | + | + | − | − | + | + | NT | NT |
| 7 | 72/M | 153 | MDS-LGL | − | − | − | − | − | − | + | − | + | − | − | − | − | − | − | − | − |
| 8 | 27/F | 88 | LYP | − | − | − | − | − | + | ND | ND | ND | − | − | + | − | ND | ND | − | − |
| 9 | 86/M | 53 | B-NHL | − | + | − | − | − | − | ND | − | ND | − | − | − | − | − | ND | + | + |
+, Clonal gene rearrangement; −, polyclonal gene rearrangement; B-NHL, B-cell non-Hodgkin lymphoma; CTCL, cutaneous T-cell lymphoma; LYP, lymphomatoid papulosis; MDS-LGL, myelodysplastic syndrome T/NK-cell large granular lymphocytosis; ND, not done because of insufficient DNA; NT, not tested; T/NK L, T/natural killer cell lymphoma. Anatomic sites: skin (patients 1, 2, 3, 4, 8, 9); peripheral blood (patients 5, 7); nasal sinus (patient 6). Fixation: formalin-fixed paraffin-embedded tissue (patients 1, 2, 3, 4, 6, 8, 9); fresh or frozen (patients 5, 7).
PCR master mix used as provided by the manufacturer (Mg2+ concentration not disclosed by the manufacturer).
Addition of 1 μl 25 mmol/L MgCl2 per 25 μl PCR reaction to the commercial multi-V master mix.
Two peaks three times above the background.