Table 4.
Thermodynamic Properties of TCR-γ Primers
| Primer set | Gene segment | Primer sequence | Tm (°C) | GC (%) | PCR product size range (bp)* |
|---|---|---|---|---|---|
| Mono-V | V1-8 | 5′-TACATCCACTGGTACCTACACCAG-3′ | 59.6 | 50 | 240–270 |
| V9 | 5′-GAAAGGAATCTGGCATTCCGTCAG-3′ | 59.6 | 50 | 160–200 | |
| V10 | 5′-AAGCAACAAAGTGGAGGCAAGAAAG-3′ | 57.9 | 44 | 145–175 | |
| V11 | 5′-AGTAAAAATGCTCACACTTCCACTTC-3′ | 56.4 | 38.5 | 130–160 | |
| Range | 56.4–59.6 | 38.5–50 | |||
| JP1/JP2 | 5′-GAAGTTACTATGAGCTTAGTCCCTT-3′ | 56.3 | 40 | ||
| J1/J2 | 5′-TACCTGTGACAACAAGTGTTGTTC-3′ | 56.2 | 41.7 | ||
| JP | 5′-AAGCTTTGTTCCGGGACCAAATAC-3′ | 57.9 | 45.8 | ||
| Range | 56.2–57.9 | 40–45.8 | |||
| Multi-V | V1-8 | 5′-GGAAGGCCCCACAGCRTCTT-3′ | 62.5 | 64.3 | 200–255 |
| V9 | 5′-CGGCACTGTCAGAAAGGAATC-3′ | 57.6 | 52.4 | 155–225 | |
| V10 | 5′-AGCATGGGTAAGACAAGCAA-3′ | 53.4 | 45 | 145–200 | |
| V11 | 5′-CTTCCACTTCCACTTTGAAA-3′ | 51.3 | 40 | 90–110† | |
| Range | 51.3–62.5 | 40–64.3 | |||
| JP1/JP2 | 5′-TTACCAGGCGAAGTTACTATGAGC-3′ | 57.4 | 45.8 | ||
| J1/J2 | 5′-GTGTTGTTCCACTGCCAAAGAG-3′ | 57.7 | 50 | ||
| Range | 57.4–57.7 | 45.8–50 |
*
Mono-V and multi-V size ranges based on measuring PCR products that could be amplified from DNA extracted from 10 reactive tonsils.
†
DNA from only 1/10 reactive tonsils could be amplified using the multi-V V11 primer set.