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Gel electrophoresis of PCR products. IgVH fragments from eight CLL samples (each positive for a different VH gene family and the VH3-21 segment) were amplified. PCR reactions with the consensus leader primers for the VH1, VH3, or VH4 gene families are labeled 1, 3, and 4, respectively. The multiplexed PCR reaction combining the VH2, VH5, and VH6 consensus leader primers and the VH3-21 leader primer is labeled C. Fragment sizes are indicated in bp.
