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Effect of combining TaqMan and SYBR Green chemistries in single real-time PCRs. M. tuberculosis CDC 1551 target at 107 gene copies and two 100-fold serial dilutions were tested. Dotted lines represent reactions containing both TaqMan and SYBR Green, whereas solid lines represent reactions containing either SYBR Green (A) or TaqMan (B) alone. A shows 16S SYBR Green signals, and B shows RD TaqMan Cy5 signals.
