Table 3.

Potential of the Combined 16S-RD Assay to Detect Mixed Infections

	M. smegmatis (16S SYBR Green signal)			M. tuberculosis CH (RD SYBR Green signal)
	105*	104	103	105	104	103
M. tuberculosis CDC 1551 signals†
105	BOTH‡	CDC	CDC	BOTH	CDC	CDC
104	BOTH	BOTH§	CDC	BOTH	BOTH	CDC
103	MS	BOTH	CDC	BOTH	BOTH	BOTH

^*Figures denote number of target DNA copies added.

^†TaqMan signals from both 16S and RD assays.

^‡BOTH, both targets detected; CDC, only M. tuberculosis CDC 1551 (RD750⁺) detected; MS, only M. smegmatis detected. No M. tuberculosis CH (RD750⁻) only signals were obtained.

^§Borderline M. smegmatis signal.