FIG. 2.

Effect of PBDE congeners on OATP-mediated uptake of known substrates in stably transfected CHO cells. In the absence or presence of BDE47, BDE99, or BDE153 at the indicated concentrations, uptake of 1μM [³H]estradiol-17-β-glucuronide was measured at 37°C for 20 s with (A) OATP1B1- or (B) OATP1B3-expressing and wild-type CHO cells, while uptake of 1μM [³H]estrone-3-sulfate was measured at 37°C for 30 s with (C) OATP2B1- or pcDNA5/FRT-expressing CHO cells. Values obtained with wild-type or vector-transfected CHO cells were subtracted from values obtained with OATP-expressing CHO cells and are given as percent of the control. Means ± SE of triplicate determinations are given. Differences were considered significant at p < 0.05.