FIG. 3.

Time- and concentration-dependent uptake of BDE47 by OATP1B1, OATP1B3, and OATP2B1. (A) OATP1B1-, (B) OATP1B3-, or (C) OATP2B1-mediated uptake of [¹⁴C]BDE47 at 37°C at the indicated time points. Filled circles (•) represent control (wild-type CHO; pcDNA5/FRT CHO) uptake, while open circles (○) represent OATP1B1, OATP1B3, or OATP2B1 uptake. Determination of kinetic parameters was performed in CHO cells expressing OATP1B1, OATP1B3, or OATP2B1. Uptake of increasing concentrations of [¹⁴C]BDE47 was measured at 37°C for 30 s. After subtracting the values of the control (•) from OATP-expressing cells (○), net (D) OATP1B1-, (E) OATP1B3-, or (F) OATP2B1-mediated uptake data were fitted by nonlinear regression analysis to the Michaelis-Menten equation and plotted as a dashed line (- - -). Means ± SE of triplicate determinations are given. The unpaired Student’s t-test was performed to determine statistical significance. Differences were considered significant at p < 0.05.