TABLE 1.

Kinetic Parameters of OATP1B1-, OATP1B3-, and OATP2B1-Mediated Uptake of PBDEs in CHO Cells

Transporter	Substrate	Km (μM)	Vmax (nmol/mg protein × min)	Vmax/Km
OATP1B1	BDE47	0.31 ± 0.01	13.5 ± 1.33	44.0 ± 2.8
	BDE99	0.91 ± 0.02	13.9 ± 1.70	15.4 ± 2.2
	BDE153	1.91 ± 0.17	2.4 ± 0.19	1.2 ± 0.02
OATP1B3	BDE47	0.41 ± 0.06	15.7 ± 1.71	38.0 ± 1.1
	BDE99	0.70 ± 0.03	12.8 ± 0.76	18.3 ± 0.1
	BDE153	1.66 ± 0.48	22.6 ± 0.58	1.5 ± 5.3
OATP2B1	BDE47	0.81 ± 0.06	17.6 ± 1.1	22.6 ± 1.1
	BDE99	0.87 ± 0.22	8.9 ± 0.9	10.5 ± 1.6
	BDE153	0.65 ± 0.07	14.6 ± 3.1	28.1 ± 8.1

Note. Transport rates at increasing concentrations were determined at 37°C in OATP-expressing and wild-type CHO or vector-transfected cells. Transport values obtained from OATP-expressing cells were corrected with values obtained from wild-type cells, and the resulting net carrier-mediated uptake values were fitted by nonlinear regression analysis to the Michaelis-Menten equation. Means ± SE are given for three to four experiments.