FIG. 4.

Effect of NaNO₂ on cyanide inhibition of cellular oxygen consumption. Representative oxygen consumption as determined by Clark-type oxygen electrode showing (A) inhibition by KCN (20μM) and lack of effect of PTIO; (B) reversal of KCN inhibition in cells pretreated with NaNO₂ (200μM) and reversal of NaNO₂ action by pretreatment with PTIO. (C) Effect of PTIO and NaNO₂ on cyanide inhibition of oxygen consumption. Cells were pretreated with NaNO₂ (200μM) for 10 min and then KCN (20μM) and PTIO (200μM) were added. Data represent the mean ± SEM of three or more experiments. *Significantly different from untreated control group; ^#significantly different from KCN treatment group; ^&significantly different from NaNO₂ + KCN treatment group. p < 0.05.