Skip to main content
. Author manuscript; available in PMC: 2010 May 17.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2009 Feb 14;50(7):3283–3290. doi: 10.1167/iovs.08-3052

Figure 5.

Figure 5

αB-crystallin in P20 rat lens. Postnuclear homogenates obtained from three different regions of P20 rat lens (inset) were fractionated as described. Note that for E+SC, 524 μg total protein was loaded on the gradient compared with 8.40 mg and 10.00 mg for BR and FM, respectively. Thus, the BR and FM fractions had to be diluted 20× so that similar amounts of protein could be analyzed. Comparable amounts of protein (E+SC, 26.2 μg/fraction; BR, 21 μg/fraction; FM, 25 μg/fraction) were loaded on the gel to follow the distribution of αB-crystallin. A large proportion of αB-crystallin fractionates with the Golgi-enriched membrane in all three gradients (fractions 9–12, represented by the dotted line in the panel marked E+SC). Top: whole immunoblot with molecular mass standards. Only relevant areas are shown for the BR and FM panels.