Figure 6. RT-PCR assays for the expressions of M. tuberculosis relBE-like proteins in M. smegmatis mc² 155.

The experiments and assays were performed as described in the “Materials and Methods”. Target genes and their host recombinant strains were demonstrated on the left of panel in the figure. The appropriate sites of each pair of RT-PCR primers were indicated by arrows. The RT-PCR products were detected on 1.5% agarose gel as shown on the right of the panel. Lane 1, positive control (the total DNA of each recombinant strain was used a template for PCR); lane 2, negative control (the cDNA of recombinant strain YM0 was used a template for PCR); lane 3, RT-PCT products (the cDNA of each recombinant strain was used a template for PCR).