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. 2010 May 17;5(5):e10665. doi: 10.1371/journal.pone.0010665

Figure 4. Morpholino knock down of kermit2 inhibits gastrulation and FN matrix assembly.

Figure 4

(A–C) Ventral view of stage 12 embryos. (D–F) Late gastrula stage BCR stained for FN. (A) Stage 12 control embryos gastrulate normally and (D) assemble a dense FN matrix. Embryos injected with 20 ng of Kermit2 5 mismatch control morpholino (COMO; B) gastrulate normally, and (E) exhibit a small decrease in FN matrix assembly. (C) Embryos injected with kermit2 inhibiting morpholino (MO) exhibit delayed blastopore closure and (F) a sparse FN matrix network. (G) Western blot of kermit2 expression. Kermit2 is expressed throughout early development. (H) Morpholino knock down of kermit2 in stage 11 embryos. 30 ng of control morpholino (CO 30) has no effect on Kermit2 expression. The Kermit morpholino at 10 ng and 20 ng (MO 10 and MO 20) decreases kermit2 expression below control levels. Molecular mass is indicated to the left of the panel. (A–C) size marker  = 200 µm. (D–F) size marker  = 30 µm.