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. 2010 May 17;5(5):e10665. doi: 10.1371/journal.pone.0010665

Figure 5. Expression of kermit2 mRNA rescues morpholino knock down of kermit2.

Figure 5

(A–D) Ventral view of stage 12 embryos. (E–H) Late gastrula stage BCR stained for FN. (A) Stage 12 control embryos gastrulate normally and (E) assemble a dense FN matrix. Embryos injected with 10 ng of Kermit2 morpholino (MO; B) gastrulate normally, and (F) exhibit a small decrease in FN matrix assembly. (C) Embryos injected with kermit2 inhibiting morpholino and kermit2 mRNA (MO+kermit2) exhibit a small delay in blastopore closure and (G) a partial rescue of FN matrix assembly. (D) Embryos injected with the kermit2 morpholino and the dominant negative kermit2 construct (MO+kermit2mut) exhibit delayed blastopore closure and (H) significant reduction in FN matrix assembly. (A–D) size marker  = 200 µm. (E–H) size marker  = 30 µm.