Figure 7. Cell adhesion assays.

(A–C) Spider graphs representing migration tracks of individual cells plated on FN substrates. Each graph contains 4 representative tracks with the start point set to (0,0). Horizontal and vertical scale is in µM. (C) Kermit2_mut expressing cells have reduced migration paths as compared to (A) control and (B) kermit2 expressing cells. (D) Quantification of activin-treated cell adhesion to FN substrates. Cells were plated on FN substrates and counted pre-wash (blue) and after washing to remove non-adherent cells (red). Control, kermit2, and kermit2_mut expressing cells all show similar affinity for FN substrates. (E) Activin-treated cell migration rate mediated by kermit2. Average cell migration velocities were measured using the track cells function of Openlab. The value for each construct represents the average (±SD) of 45 cells from three spawnings. Cells expressing the kermit2_mut construct (0.6 µM/hr) migrate significantly (P<0.005) slower than control (91 µM/hr) or kermit2 (97 µM/hr) expressing cells. (F) The radial displacement of activin-treated cells on FN substrates. Measurements are from the same cells as represented in (E). Kermit2_mut expressing cells travel significantly (P<0.01) less distance (32 µM) than control (148 µM) or kermit2 expressing cells (147 µM).