Figure 11. Colocalization of α5β1, kermit2, and Rab 21 in Xenopus XTC cells.

XTC cells were stained for α5β1 (green), or kermit2 (red D), or Rab 21 (red H). (A, E) DIC images of adherent cells, boxes represent areas magnified in B–D and F–H. (A–D) Integrin (C; green) and kermit2 (D; red) colocalize in numerous vesicles and at focal adhesions (arrowheads in B–D). (E–H) Rab 21 (H; red) and integrin (G; green) colocalize in cytoplasmic vesicles and focal adhesions (arrowheads in F–H). Staining of cells with secondary antibodies alone produced no detectable signal. Size marker  = 25 µM.