Skip to main content
. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: Virology. 2010 Apr 8;402(1):121–128. doi: 10.1016/j.virol.2010.03.014

Figure 1. EBV Rta regulates IFN-β promoter activity.

Figure 1

A) 293T cells were transfected with EBV Rta or vector control along with IFN-β reporter plasmid and Renilla luciferase plasmid. Cells were mock-infected or infected with Sendai virus (50 HA units/ml) 16 hours post transfection. Eight hours later (24 hours post transfection), cells were harvested for firefly luciferase assays and normalized to Renilla luciferase expression. B) 293T cells were transfected with EBV Rta or vector control and Sendai virus infected 16 hours post transfection. 24 hours post transfection, cells were collected and RNA harvested for semi-quantitative RT-PCR to examine IFN-β and GAPDH expression. 293T cells were transfected with the vector control or C) IRF3 or D) IRF7 in the presence or absence of EBV Rta.or the vector control and luciferase assays were performed 24 hours post transfection. Data are shown as the fold change (relative to vector control) ± standard deviation of experiments performed in triplicate.