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. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: Virology. 2010 Apr 8;402(1):121–128. doi: 10.1016/j.virol.2010.03.014

Figure 2. EBV Rta negatively regulates IRF3 and IRF7 protein expression but does not alter IRF5 expression.

Figure 2

293T cells were transfected with Flag-IRF3, Flag-IRF5, Flag-IRF7, and/or Flag-EBV Rta as indicated. A) 48 hours post transfection whole cell lysates were collected and Western blot analyses performed examining Flag expression/exogenous protein expression. B) 48 hours post transfection, cytoplasmic extracts and nuclear extracts were collected and Western blot analyses performed examining Flag expression in the two extracts. Tubulin and lamin were used as loading controls.