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. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: Virology. 2010 Apr 8;402(1):121–128. doi: 10.1016/j.virol.2010.03.014

Figure 5. Endogenous IRF3 and IRF7 RNA and protein expression is reduced during EBV lytic infection.

Figure 5

Viral reactivation was performed in Akata cells where the cells were incubated with F(AB′)2 fragment to human IgG for 0 hours (0 h), 24 hours (24 h), or 48 hours (48 h). A) Total RNA was harvested and semi-quantitative RT-PCR performed examining IRF3, IRF5, IRF7, and Rta RNA expression. B) Whole cell lysates were collected and Western blot analyses performed examining IRF3, IRF5, IRF7, Rta, and EA-D protein expression. Densitometry was performed in which relative RNA or protein expression was normalized to relative GAPDH expression. Data are shown as fold change (relative to 0 hour post induction) ± standard deviation of experiments performed in triplicate.