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. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: Virology. 2010 Apr 8;402(1):121–128. doi: 10.1016/j.virol.2010.03.014

Figure 6. Endogenous IFN-β RNA levels decrease during EBV lytic infection and coincide with increased susceptibility to Sendai virus infection.

Figure 6

Viral reactivation was induced in Akata cells as in Figure 5. A) Total RNA was harvested and semi-quantitative RT-PCR performed to detect IFN-β and GAPDH RNA at 0, 24, and 48 hours post induction. Densitometry was performed where relative IFN-β levels were normalized to relative GAPDH levels. Data are shown as the fold change (relative to 0 hour post induction) ± standard deviation of experiments performed in triplicate. B) 24 hours after induction (or mock-induction), Akata cells were infected with Sendai virus (50 HA units/ml). 24 hours after infection, supernatant fluids were collected for plaque assays on LLC-MK2 cells. Viral titers were determined and data are shown as PFU/ml ± standard deviation of experiments performed in triplicate.