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Immunolabeling with pan Shank antibody in cultured hippocampal neurons showing representative distribution of label at rest (A), after two minutes of high K+ (B), and after 30 min of recovery from high K+ (C). The density of label in the PSD and contiguous network (Zone I) appears to increase with stimulation, and return to resting level with 30 min rest. Scale bar = 100 nm.
