Fig. 3.

Immunohistochemistry for DAN. At week 1, the fractured bone is not stained with DAN (A). In weeks 2 (B) and 3 (C), DAN is strongly stained in the newly formed woven bone, but not in the mature lamellar cortical bone. DAN is stained with Texas Red, red. Nuclear is counterstained with DAPI, blue. C: cortical bone; W: woven bone; bar = 100μm.