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Mutations of putative DNA binding sites of transcription factors
| Recognition | Location | Orientation | DNA sequence | Restriction site | |
|---|---|---|---|---|---|
| WT | Mutated | ||||
| MYB 1AT | −1362 | + | ATAACCA | CTCGAGA | XhoI |
| MYC | −1358 | ± | CAATTG | TCTAGA | XbaI |
| MYB ST1 | −1228 | − | TATCCT | GGATCC | BamHI |
| napA | −1136 | + | CAAACAC | CCTCGAG | XhoI |
DNA sequences of putative DNA binding motifs (in bold) were mutagenized into a sequence containing restriction site (underlined)
