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. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: Virology. 2010 Apr 8;402(1):102–111. doi: 10.1016/j.virol.2010.02.031

Figure 5.

Figure 5

Transfer of VP3159–166 specific T cell blasts protects SJL mice from CNS demyelination and CD4+ T cell infiltration. SJL mice were infected with TMEV on day 0, then mice were injected i.v. with PBS (A, C) or 2×106 VP3159–166-specific T cell blasts (B, D) on day 2 p.i. On day 75 p.i., 5 mice per group were sacrificed and brain and spinal cord tissues were harvested and frozen in liquid nitrogen. Sections were cut from frozen tissues and stained with a fluoromyelin stain (green) and anti-CD4 (red), then counterstained with DAPI (blue). Representative cerebellum (A, B) and lumbar spinal cord (C, D) tissue sections are shown. The PBS treated mouse shown was at a score of 3 at the time of sacrifice. Both CD4+ cells and a lack of myelin staining are evident in both cerebellum and spinal cord tissue (A, C). The VP3159–166 T cell blast recipient pictured was at a score of 0 and shows intact myelin and a lack of CD4+ T cell infiltration (B,D).