VP3159–166-specific T cell blasts are retained in the CNS of TMEV-infected, but not mock-infected, SJL mice. VP3159 blasts were labeled with 2μM CFSE prior to transfer into SJL mice that were mock-infected or infected with TMEV 2 days previously. 2 days post-transfer (day 4 p.i.), mice were sacrificed and spleen, liver, lung, and brain tissues were harvested, and infiltrating cells analyzed by flow cytometry for CFSE+ cells. For each histogram, organs from 3 mice per group were pooled. Data are representative of 3 separate experiments.