Skip to main content
. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: Virology. 2010 Apr 7;402(1):83–93. doi: 10.1016/j.virol.2010.03.017

Fig. 3. A33 is required for proper targeting of B5-GFP.

Fig. 3

HeLa cells were infected with the indicated viruses at a MOI of 1.0 and transfected with the indicated plasmids. 24 h PI, cells were fixed, permeabilized, and A33-HA was visualized by staining with an anti-HA MAb, followed by Cy-5-labeled donkey-anti-rat antibody (Red). Inserts show HcRed fluorescence (Orange), indicating that cells were transfected and infected. Localization of B5-GFP or A33-HA at the site of wrapping (concave arrowheads), at the vertices (arrows), and on virion-sized particles (arrowheads) is indicated. The DNA in nuclei and viral factories was stained with DAPI (Blue). B5-GFP is shown in green. The overlap of green and red is shown in yellow.