U87 indicator cells expressing only CD4, CD4 and CCR5, or CD4 and CXCR4 were infected with HIV-1 luciferase reporter viruses pseudotyped with primary isolate envelopes (C2, DR, NR), prototype R5X4 envelopes (89.6, DH12 or R3A), R5 (Bal or JRFL) or X4 envelopes (Tybe) as controls. Three days later, infection levels were determined by measuring luciferase activity in cell lysates. The results are expressed in relative light units (RLUs) and represent means +/- standard error (SEM) for two experiments performed in triplicate.