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. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: Virology. 2010 Apr 9;402(1):135–148. doi: 10.1016/j.virol.2010.03.009

Figure 2. CXCR4 is the principal coreceptor on PHA/IL-2 stimulated CD4+ lymphocytes for R5X4 HIV-1 primary and prototype strains.

Figure 2

CD4+ T cells were stimulated with PHA for 3 days then treated for 1 hour before infection with or without the CXCR4 blocker AMD3100 (5μg/ml; “CCR5 pathway”), CCR5 blocker M657 (5μM; “CXCR4 pathway”) or both (“neither pathway”). Cells were then infected with HIV-1 luciferase reporter viruses in the continued presence of coreceptor blockers and maintained with IL-2 (10 U/ml). Four days post infection, viral entry was determined by luciferase activity in cell lysates. Results shown for each virus are presented in RLUs (A) and as a percentage of the RLUs seen in the absence of coreceptor blockers (B). Data are means +/- SEM of three experiments using lymphocytes from different donors, each done in duplicate.